* Chromatography Lab Report
* Chromatography is a useful method for separating the substances in certain mixtures. There are many physical processes which may be used to separate mixtures. In our lab we used a mobile phase, stationary phase, and retention time. Because the substances which make up certain mixtures dissolve at different rates, these substances can be separated by chromatography.
* We Thought that H2O would be the best mobile phase because it has the least amount of hydrogen, the lower the hydrogen the better the mobile solvent
Materials:
* We tested the solvent(or mobile phase) - H2O(water), CH3OH(Methanol),C3OH(Isopropyl),C6H14(Hexane)
*** BE SURE TO CARRY OUT ALL SAFETY PROCEDURES: SAFETY GOGGLES, APRON, KEEP FACE AWAY FROM SOLVENTS, AND TRY TO KEEP THE SOLVENTS UNDER THE FUME HOOD!!!
* First we took a black marker and dotted our test strips or chromatography strips approximately 1.5 to 2 inches from the bottom( the strips should be approximately 1 cm X 8 cm.) We also used four different colors just to see the turn out.Then fill 4 separate wells on the 24 well plate approx. 1/2 full of the following solvents: H2O, CH3OH, C3H7OH, and C6H14. Then place your paper strips into the wells so that the short end is in the solvent. Allow the solvent to wick up the paper for approx. 1/2 hour. Be sure to not let the dots touch the solvent!
Here is an appropriate picture of what your lab should look like:
*We found that H2O would be the best mobile phase among these four solvents.
* Here are our notes including a data table, and information on our lab report and additional notes made during the lab.
*We accepted our hypothesis because we ended up being correct about water being the best mobile phase. In our lab we found out that H2O had the greatest mobile phase and then methane was next with the second highest mobile phase, then was Isoproplyn, and the one with the lowest mobile phase was hexane. Chromatography is a method that helps scientists, law enforcement, and even schools and companies determine the composition of a particular sample. Basically, scientists need a method to separate organic from inorganic compounds. Chromatography has promptly developed as an important tool for the tricky job of analyzing materials and samples of unknown mixtures.
***If the solvent hit the dots you would have to restart your chromatography strips. Make sure to label the solvents correctly knowing which solvent is in each well. These are the tiny errors we had during our chromatography lab
* Chromatography is a useful method for separating the substances in certain mixtures. There are many physical processes which may be used to separate mixtures. In our lab we used a mobile phase, stationary phase, and retention time. Because the substances which make up certain mixtures dissolve at different rates, these substances can be separated by chromatography.
* We Thought that H2O would be the best mobile phase because it has the least amount of hydrogen, the lower the hydrogen the better the mobile solvent
Materials:
* We tested the solvent(or mobile phase) - H2O(water), CH3OH(Methanol),C3OH(Isopropyl),C6H14(Hexane)
*** BE SURE TO CARRY OUT ALL SAFETY PROCEDURES: SAFETY GOGGLES, APRON, KEEP FACE AWAY FROM SOLVENTS, AND TRY TO KEEP THE SOLVENTS UNDER THE FUME HOOD!!!
* First we took a black marker and dotted our test strips or chromatography strips approximately 1.5 to 2 inches from the bottom( the strips should be approximately 1 cm X 8 cm.) We also used four different colors just to see the turn out.Then fill 4 separate wells on the 24 well plate approx. 1/2 full of the following solvents: H2O, CH3OH, C3H7OH, and C6H14. Then place your paper strips into the wells so that the short end is in the solvent. Allow the solvent to wick up the paper for approx. 1/2 hour. Be sure to not let the dots touch the solvent!
Here is an appropriate picture of what your lab should look like:
*We found that H2O would be the best mobile phase among these four solvents.* Here are our notes including a data table, and information on our lab report and additional notes made during the lab.
*We accepted our hypothesis because we ended up being correct about water being the best mobile phase. In our lab we found out that H2O had the greatest mobile phase and then methane was next with the second highest mobile phase, then was Isoproplyn, and the one with the lowest mobile phase was hexane. Chromatography is a method that helps scientists, law enforcement, and even schools and companies determine the composition of a particular sample. Basically, scientists need a method to separate organic from inorganic compounds. Chromatography has promptly developed as an important tool for the tricky job of analyzing materials and samples of unknown mixtures.
***If the solvent hit the dots you would have to restart your chromatography strips. Make sure to label the solvents correctly knowing which solvent is in each well. These are the tiny errors we had during our chromatography lab
